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庞巧兰[1] 李庆刚[2] 石艳丽[3][1]山东鲁抗医药集团公司,山东济宁272021 [2]山东大学发酵工程国家重点实验室,山东济南250100
[3]山东福瑞达生物化工有限公司,山东济南250014摘 要:目的 改进青霉素发酵罐的接种工艺，提高青霉素生产水平。方法
采用不同接种量、不同接种方式的对照实验和正交化实验，改进接种工艺和与之匹配的发酵前期工艺。结果 采用两种种源混合接入，总接种量为30％，其中种罐种子液的接种量为20％，发酵至60h的前期发酵液的接种量为10％。结论接种工艺改进后，发酵指数提高10．8％，提炼收率提高0．4％，发酵提炼的生产指数提高11．2％。[著者文摘]
关键词:青霉素 发酵罐 种罐 接种量
分类号: R978.11[著者标引]文献标识码：A文章编号：1672-979X（2005）09-0026-03栏目信息：论著
相关文献:主题相关
Improved inoculation of peniciUion fermenterPang QiaoLan;Li
QingGang;Dan YanLiPang QiaoLan;Li QingGang;Dan YanLiAbstract:Objective
To improve inoculation of penicillion fermenter and to increase
level of penicillion production. Methods Inoculation and
accordant earlier fermentation process were optimized by
comparison and orthogonal design experiments. Results It
was adopted that overall inoculation volume of two types
seeds was 30%, respectively, the inoculation of seed broth
was 20% and the one of fermentation broth of 60th hour was
10%. Conclusions It was showed that fermentation index was
increased by 10.8%, refinement yield 0.4%, and production
index of fermentation and refinement 11.2%.[著者文摘]
Key words:penicillin; fermentor; seed tank; inoculation
volume
收稿日期: 2005-05-31修订日期: 基金资助:
作者简介:庞巧兰（1972-），女，山东济宁市人，硕士研究生，主要从事生物制药研究。通讯作者：石艳丽，女，主管药师，Tel：053l-88937780-1530。 |
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