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黄国昌[1] 熊妮[2] 印培民[2] 黄筱萍[2][1]南昌大学生命科学院,江西南昌330047 [2]江西省科学院,江西南昌330029摘 要:在50L自控式发酵罐上进行了ε-聚赖氨酸（ε-PL）发酵条件的优化研究，主要考察了分批发酵和补料分批发酵过程中pH、搅拌转速对ε-PL发酵和菌体生长的影响。结果表明，当控制pH在5．0以上时有利于菌体的生长，但ε-PL基本不合成，甚至出现降解现象；当维持pH在4．0左右时，能促进ε-PL的合成。搅拌转速为300r／min时，有利于溶氧和传质，400r／min时，由于剪切力过大，导致菌丝断裂，ε-PL产量下降。通过控制发酵中后期pH4．0和搅拌转速300r／min的pH反馈控制自动流加补料培养，可获得最大的ε-PL产量7．36g／L，产率0．072g／g，产量较优化前提高近10倍，产率提高2倍。[著者文摘]
关键词:ε-聚赖氨酸（ε-PL） 白色链霉菌 发酵 补料 pH控制
分类号: TS210[机标]文献标识码：文章编号：栏目信息：研究报告
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Optimization of the ε-Poly-L-lysine Fermentation Conditions
in 50 L FermentorHuang Guochang , Xiong Ni, Yin Peimin,
Huang Xiaoping1.College of Life Sciences, Nanchang University,
Nanchang 330047, China; 2;Jiangxi Academy of Sciences, Nanchang
330029, ChinaAbstract:In order to optimize the ε-poly-L-lysine（ε-PL）
fermentation conditions in a 50 L fermentor, the effects
of pH and stirrer speed on the batch fermentation and fed-batch
fermentation of ε-PL production were studied. The results
showed that the growth of cells was promoted when pH remained
at 5.0, the synthesis of ε-PL was inhibited, the accumulated
ε-PL was depolymerized, and high ε-PL accumulation was achieved
at pH pH4. 0. Under the optimal condition, controlling pH4.0
by glucose auto-feeding and stirrer speed 350 r/min at later
stage, the production of ε-PL was 7.36g/L, the yield to
glucose was 0. 072g/g. This con- trol and feeding strategy
enhanced the production of ε-PL 11 times.[著者文摘]
Key words:ε-poly-L-lysine（ε-PL）, Streptomyces albulus,
fermentation, fed batch, pH control
收稿日期: 2007-04-10修订日期: 2007-06-18基金资助:
作者简介:硕士研究生。 |
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