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高年发 苏敬红 张健天津市工业微生物重点实验室,天津科技大学生物工程学院,天津300022摘 要:对L-嗜热乳酸杆菌T-1在5L发酵罐上进行了分批发酵和补料分批发酵的条件研究。实验结果表明：分批发酵最适条件为以Na2CO3作中和剂，温度50℃；pH6．0，溶氧范围为前0h～20h通风1L/in，搅拌转速100r／min，20h后停止通风和搅拌，产乳酸95g/L，产酸速率1．32g／（L·h），转化率92．7％。补料分批发酵实验表明，2次补料乳酸产量明显高于1次补料，产酸117．5g／L，转化率90．4％。[著者文摘]
关键词:L-乳酸 发酵 嗜热乳酸杆菌
分类号: TQ921[著者标引]文献标识码：B文章编号：0254-5071（2006）03-00011-03栏目信息：研究报告
相关文献:主题相关 全文快照
Study on L-Lactobacillus thermophilus T- 1 fermentation
in 5L fermenterGAO Nian-fa, SU Jing-hong, ZHANG Jian Tianjin
Key Laboratory of Industrial Microbiology, College of Bioengineering,
Tianjin University of Science ＆ Technology, Tianjin 300222,China
Abstract:The conditions of batch and fed-batch fermentation
by L-Lactobacillus thermophilus T-1 in a 5 L fermenter were
studied. The production yield of lactic acid reached 95
g/L with a rate of 1.32 g/（L h）, and a conversion rate of
92.7% with conditions ofpH 6.0, temperature 50℃ and the
dissolved oxygen controlled by aeration 1 L/min and stirring
100 r/min for 20 h. Furthermore, this article also shows
that two times fed-batch was better than one time fed-batch,
the production amount of lactic acid was up to 117.5 g/L
and the conversion rate was 90.4%.[著者文摘]
Key words:L-lactic acid; fermentation; Lactobacillus thermophilus
收稿日期: 2005-11-07修订日期: 2005-12-06基金资助:
作者简介:高年发（1945-），男，江苏吴江人，教授，主要研究有机酸发酵。 |
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