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孙丽艳[1] 崔洪斌[1] 赵琪[2][1]哈尔滨医科大学公共卫生学院,哈尔滨150086 [2]河北省廊坊市防疫站,廊坊065000摘 要:纳豆菌1号（BJC—1）是一株高产大豆异黄酮糖苷转化酶的菌株。本实验采用正交设计的方法，筛选出该菌的最佳产酶条件为：2％的葡萄糖为碳源；2％的大豆蛋白胨为氮源；发酵液pH值维持在7．0-7．5之间；装液量为2L；接种量为6％；发酵温度34℃；转速180r／min；发酵时间为32h。其产酶的最高酶活可达127．60IU。Ca^2＋、Mg^2＋对产酶有促进作用；Cu^2＋、Fe^3＋对产酶有抑制作用；K^＋、Zn^＋对产酶基本没有影响。[著者文摘]
关键词:大豆异黄酮 糖苷转化酶 纳豆菌 发酵条件
分类号: S565.1[免标]文献标识码：A文章编号：1009-2765（2006）03-0017-05栏目信息：病虫害识别与防治
相关文献:主题相关
Study on The Fermentation Conditions of Soy Isoflavone
Glycosidase Produced by FermentorSun Liyan, Cui Hongbin,
Zhao Qi 1. Public Health Institute of Haerbin Medical University,
Harbin 150086; 2. Langfang Health and Epidemic Provention
Station, Langfang 065000Abstract:BJC - 1 is a natto strain
produced high enzymatic activity of soy isoflavone glycosidase
. The optimal conditions of its produein β - glycosidase
were explored by us applying orthogonal experiment. The
results demonstrated that the total volume of fermentation
broth is 2L and the concentration of glucose, soy protein
and seeds are 2%, 3% and 6% respectively. The optimal pH
is 7.0 - 7.5 and the temperature, rotation speed and time
are 34℃, 180 r/min and 32 h. Ca^2＋ and Mg^2＋ will promote
the process of fermentation, while Cu^2＋ and Fe^3＋ will
inhibit it. K^＋ and Zn^2＋ have no effeet on it. The highest
enzymatic activity of β- glyeosidase is 127.60 IU.[著者文摘]
Key words:soy isoflavone, soy isoflavone glycosidase, bacillus
natto, fermentable condition
收稿日期: 2006-02-22修订日期: 基金资助:科技部十五重大专项“大豆深加工关键技术及设备研究与开发”,编号：2001BA501A02.
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