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陈祥仁[1] 黄霄红[2] 徐宝多[3] 卢英华[3][1]厦门大学生命科学学院,福建厦门361005 [2]厦门大学医学院药学系,福建厦门361005
[3]厦门大学化学化工学院化学工程与生物工程系,福建厦门361005摘 要:利用SIH合成培养基，在7L发酵罐培养盘基网柄菌．培养147h后，细胞密度达到4．0×10^7mL^-1，为在复杂培养基上所能达到的细胞密度的2～4倍．培养过程中葡萄糖的消耗量为6．7g／L，产氨浓度达0．88g／L．对培养基中的氨基酸分析表明，赖氨酸、色氨酸、甲硫氨酸和苯丙氨酸消耗较快，显示SIH培养基的氨基酸成分还可进一步优化．采用基于Monod生长动力学的半经验模型可很好模拟细胞生长和底物消耗，并估计出动力学参数μmax=0．115h^-1，Nmax=6．0×10^7mL^-1．本研究为进一步优化合成培养基和为利用这一新型真核表达系统大规模生产重组异源蛋白奠定了基础．[著者文摘]
关键词:盘基网柄菌 合成培养基 发酵罐 培养
分类号: Q939.97[免标]文献标识码：A文章编号：0438-0479（2006）S-0032-05栏目信息：生命科学
相关文献:主题相关
High Cell Density Cultivation of Dictyostelium in Conventional
BioreactorCHEN Xiang-ren, HUANG Xiao-hong, XU Bao-duo, LU
Ying-hua 1. School of Life Science, Xiamen Univ. , 2. Department
of Phannaceutics, School of Medicine, Xiamen Univ. , 3.
Department of Chemical and Biochemical Engineering, College
of Chemistry and Chemical Engineering, Xiamen Univ. , Xiamen
361005, ChinaAbstract:Dictyostelium discoideum was cultivated
on a novel synthetic SIH medium in 7 L bioreactor. After
147 h cultivation, cell density was reached up to 4.0×10^7
mL^-1. It was about 2～4 times higher than that could be
obtained in axenic complex medium. Up to 6.7 g/L glucose
was consumed and 0.88 g/L ammonium was produced during the
cultivation, respectively. Analysis of samples with respect
to amino acids indicated that lysine, tryptophan, methionine
and phenylalanine were consumed completely after a cultivation
time of 160 h. This results showed that the composition
with respect to the amino acids might still be further optimized.
The cell growth activity and glucose consumption could be
modeled with semi-empirical balance equations based on Monod
kinetics. The estimated parameters were μmax = 0.115 h^-1,
Nmax =6.0×10^7 mL^-1. This study provided basic data for
large scale production of heterologous proteins with Dictyostelium
discoideum.[著者文摘]
Key words:Dictyostelium discoideum; synthetic medium; bioreactor;
cultivation
收稿日期: 2006-01-23修订日期: 基金资助:国家自然科学基金（20306025）资助
作者简介:陈祥仁（1969-），男，实验师．通讯作者：ylu@xmu．edu．cn |
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