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易有金 罗坤 柏连阳 刘二明 罗宽湖南农业大学生物安全科学技术学院,湖南长沙410128摘 要:烟草内生拮抗菌枯草芽孢杆菌B.001菌株在田间连续4年防治烟草青枯病均有较好的防治效果.为了提高发酵水平,节省发酵成本,使其能大规模应用于生产实际,通过正交优化试验L9(3^4)确定其5L发酵罐装液量为3L,最佳发酵条件:通气量100L/h,转速220r/min,温度30℃,起始pH8.0,发酵时间60h.[著者文摘]
关键词:烟草内生拮抗菌B-001 5 L发酵罐 最佳发酵条件
分类号: S572[著者标引]文献标识码:A文章编号:1007-1032(2007)06-0747-04栏目信息:植物保护
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The optimal fermentative condition of endophytic bacteria
B-001 in tobacco in 5 L fermentation containerYI You-jin,
LU0 Kun, B0 Lian-yang, LIU Er-ming , LU0 Kuan College of
Bio-Safety Science and Technology, HNAU, Changsha 410128,
ChinaAbstract:The endophytic inhibiting bacteria( Bacillus
subtilis)B-001 had better controlling efficacy on tobacco
Ralstonia solanacearum during four-years field test.In order
to improve the fermentation efficacy, reduce the fermentation
cost and to be practiced on bigger scale, in this paper
the optimal fermentative conditions in 5 L fermentation
container were studied. The results showed that its optimal
fermentative conditions were 100 L/h, 220 r/min, 30℃, 3
L/5 L, intial pH8.0, 60 h by orthogonal experiments L9(34).[著者文摘]
Key words:tobacco antagonistic endophyte B-001 ; 5 L fermentation
container; the optimal fermentative condition
收稿日期: 2007-05-18修订日期: 基金资助:湖南省科学技术厅重点项目(2007NK2005);湖南省教育厅重点项目(02A019)
作者简介:易有金(1968-),女,湖南涟源人,博士研究生,湖南农业大学教授.通讯作者. |