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李羿[1] 万德光[2] 杨胜[1][1]成都医学院药学系,四川成都610083 [2]成都中医药大学药学院,四川成都610075摘 要:在茯苓摇瓶液体发酵条件研究的基础上，进一步探讨了茯苓摇瓶补料液体发酵和发酵罐补料液体发酵的发酵工艺，为其产业化打下基础。通过每升发酵液中茯苓菌丝体干重的测定，确定最佳的液体发酵补料工艺。发酵罐液体补料发酵较摇瓶补料液体发酵大大缩短了发酵时间，使发酵时间从168h缩短为120h；发酵罐液体补料发酵还较摇瓶补料液体发酵提高了茯苓的生物量，使菌丝干重从10．74g／L增长到11.95g／L。[著者文摘]
关键词:茯苓 液体发酵 发酵条件 补料 发酵罐
分类号: TQ920[著者标引]文献标识码：A文章编号：1005-8915（2007）01-0056-04栏目信息：研究论文
相关文献:主题相关 全文快照
Erlenmeyer Flask Additional Medium Liquid Fermentation
and Additional Medium Liquid Fermentation in Fermentor of
Poria CocosLI Yi , WAN De-guang, YANG Sheng 1. Department
of Pharmacy , Chengdu Medical College, Chengdu 610083, China;
2. School of Pharmacy ,Chengdu University of TCM ,Chengdu
610075,ChinaAbstract:In studies on conditions of liquid
fermentation of Poria cocos, the techniques of Erlenmeyer
flask additional medium liquid fermentation and additional
medium liquid fermentation in fermentor were studied in
order to realize its industrialization in the future. Technique
of additional medium liquid fermentation was determined
through the measure of dry weight of mycelium per liter
fermentation liquid. Additional medium liquid fermentation
in fermentor could decrease the liquid fermentation time
from 168 h to 120 h and increase the dry weight of filamentous
form from 10. 74 g/L to 11.95 g /L compared with Erlenmeyer
flask additional medium liquid fermentation.[著者文摘]
Key words:Poria cocos,Liquid fermentation,Fermentation
conditions,Additional medium,Fermentor
收稿日期: 2006-05-30修订日期: 2006-07-18基金资助:致谢：感谢成都地奥制药公司的刘忠荣博士、吴洽庆博士提供了所需的实验条件和宝贵的建议.
作者简介:李羿，1969年生，男，四川成都人，助理研究员，博士，主要从事微生物发酵及中药品种、品质方面的研究。Tel：（028）81393089．E-mail：lychengdu@yahoo．com.cn
通讯作者：万德光，教授，博导。Tel：（028）87780831，E-mail：wdg@cdutcin．cdu.cn |
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