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李燕妮[1] 衣婷婷[2] 李龙森[1][1]滨州医学院基础学院,山东烟台264005 [2]山东农业大学动物科技学院,山东泰安271018摘 要:对15
L发酵罐中南极假丝酵母(C.antarctica DSM3855)的发酵工艺条件进行了研究,确定了最适的产酶工艺条件:搅拌速度为300
r·min^-1,通气量为10 L·min^-1,接种量为10%,添加GPE作为消泡剂。在此培养条件下,培养54 h产酶达到高峰值,最高酶活力达到19.3
U·mL^-1。[著者文摘]
关键词:发酵 南极假丝酵母 发酵罐
分类号: Q93-335[著者标引]文献标识码:A文章编号:1672-5425(2007)07-0043-02栏目信息:科学研究
相关文献:主题相关 全文快照
Studies on Fermentation Process for Lipase Production by
Candida Antarctica in 15 L FermentorLI Yan-ni ,YI Ting-ting
, LI Long-sen 1. College of Basic Sciences, Binzhou Medical
University, Yantai 264005, China ; 2. College of Animal
Science & Technology, Shandong Agricultural University,
Tai'an 271018,ChinaAbstract:The production of extracellular
lipase from Candida antarctica was studied in 15 L fermentor.The
optimal operation conditions were determined as follows.fermentor
agitation rate 300 r·min^-1 , ventilation -amount 10 L·min^-1
, the seeded volume 10% ,and adding GPE as antifoaming agent.
The maximum lipase activity of 19.3 U·mL^-1 was achieved
in 15 L fermentor with fermentation time 54 h.[著者文摘]
Key words:fermentation; Candida antarctica ; fermentor
收稿日期: 2007-03-15修订日期: 基金资助:滨州医学院科研启动基金项目(BY2005KYQD28)
作者简介:李燕妮(1980-),女,山西临汾人,硕士,助教,主要从事发酵工程研究。E-mail:lyn0158@126.com。 |