|
黄国昌[1] 印培民[2] 黄筱萍[2][1]南昌大学生命科学院,江西南昌330047 [2]江西省科学院,江西南昌330029摘 要:在50L自控式发酵罐上进行ε-聚赖氨酸发酵条件优化研究，主要考察了分批发酵和补料分批发酵过程中pH、搅拌转速对ε-PL发酵和菌体生长的影响。结果表明，当控制pH在5.0以上时有利于菌体的生长，但ε-聚赖氨酸基本不合成，甚至出现降解现象；当pH维持在4.0左右时，能促进ε-聚赖氨酸的合成。搅拌转速为300r/min时，有利于溶氧和传质，400r/min时，由于剪切力过大，导致菌丝断裂，ε-PL产量下降。通过控制发酵中后期pH4.0和搅拌转速300r/min的pH反馈控制自动流加补料培养，可获得最大的ε-PL产量7.36g/L，产率0.072g/g，产量较优化前提高近10倍，产率提高2倍。[著者文摘]
关键词:ε-聚赖氨酸 白色链霉菌 发酵 补料 pH控制
分类号: TS202.3[著者标引]文献标识码：A文章编号：1006-2513（2007）05-0099-05栏目信息：试验研究
相关文献:主题相关 全文快照
Optimization of the ε-Poly-L-lysine fermentation conditions
in 50L autocontrol fermentorHUANG Guo-chang, YIN Pei-min,
HUANG Xiao-ping 1. College of Life Sciences, Nanchang University
330047; 2. Jiangxi Academy of Sciences 330029Abstract:Optimization
of the ε-PL fermentation conditions in 50 L fermentor, the
effects of pH and stirrer speed on the batch fermentation
and fed-batch fermentation of ε-PL production were studied.
The results showed that the growth of cells was promoted
when pH remained at 5.0, but the synthesis of ε-PL was inhibited,
even the accumulated ε-PL was depolymerized, while high
ε-PL accumulation was achieved at pH 4. 0. Under the optimal
condition, In late stage, controlling pH4.0 by glucose auto-feeding
and stirrer speed 350r/min, the production of ε-PL was 7.
36g/L, the yield to glucose was 0. 072g/g, this control
and feeding strategy enhanced the production of ε-PL 11
times.[著者文摘]
Key words:ε-poly-L-lysine; streptomyces albulus ; fermentation;
fed batch ; pH control
收稿日期: 2007-04-07修订日期: 基金资助:
作者简介:黄国昌（1981-），男，硕士，研究方向：工业微生物学。 |
最新公告