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王付转[1] 周爱萍[2] 吴存朝[3][1]江南大学生物工程学院,江苏无锡214122 [2]平顶山教育学院生物系,河南平顶山467000
[3]漯河南街村药业集团制药有限公司,河南漯河462000摘 要:用紫外和氯化锂复合诱变利福霉素产生菌，得到发酵效价为6837u／mL的菌株A22—04，比初始菌株提高36．5％。对该菌株进行发酵罐放大试验，10L罐发酵效价为7234u／mL，发酵时间为120h，比对照菌株缩短了12h；30m^3生产罐的发酵效价为6497u／mL，发酵时间也略有缩短。[著者文摘]
关键词:利福霉素 育种 发酵罐放大
分类号: TQ330.382[机标]文献标识码：文章编号：栏目信息：研究报告
相关文献:主题相关 全文快照
Study on Breeding and Scale-up in Fermenters of Rifamycin
ProducerWang Fuzhuan, Zhou Aiping Wu Cunchao School of Biotechnology,
Southern Yangtze University, Wuxi 214122,Chin;Department
of Biology, Pingdingshan Institute of Education, Pingdingshan
467000,China;Pharmaceutical Limited, Nanjiecun Pharmaceutical
Group Corporation, Luohe 462000, ChinaAbstract:Using UV
and LiCl as mutating agent, the Amycolatopsis mediterranei
strain A22-04 was successfully obtained. The strain＇s potency
of rifamycin SV recheached 6 837 u/mL and was 36.5% higher
than the original strain. In 10 L fermentor its potency
was 7 234 u/mL and the fermentation period was 120 h ,with
12 h shorter than the former fermentation time. And in 30
m^3 tank its yield was 6 497 u/mL and the fermentation time
was little shorter than the original.[著者文摘]
Key words:rifamycin,breeding, scale-up of fermentaion
收稿日期: 2007-04-13修订日期: 2007-06-05基金资助:
作者简介:博士研究生．讲师。 |
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